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In Vitro Bioaccessibility (IVBA) and PBET
PBET apparatus

IVBA Extraction Apparatus.

PRIMA performs in vitro bioaccessibility tests for lead and according to EPA Standard Operating Procedure 9200.2-86. This method uses simulated stomach conditions to measure the amount of lead or arsenic that may be leached from ingested soil. Soil may be elevated in these compounds, but if the they are not bioavailable, then the soil may pose little risk to human health. For more information about IVBA testing, visit EPA’s IVBA FAQ page.

New Arsenic Method!  Arsenic bioaccessibility in mining-related materials is better assessed using a method developed by Ohio State University, according to California’s Department of Toxic Substances Control.  The method is similar to EPA 9200.2-86, but uses a different recipe to simulate stomach conditions and longer extraction time.  PRIMA verified the repeatability of this protocol at the request of DTSC.  Development of the method was funded by a grant from the US EPA Brownfields Research Cooperative Agreement (TR – 83415101-5).

PRIMA also performs physiologically based extraction tests (PBETs), which assess leachability of metals from ingested soil under simulated stomach and intestinal conditions. The method was developed by Ruby et al (Environ. Sci. Technol., 1996, 30, 422-430) and the stomach phase test is the basis of the IVBA test.